Fluorescence Microscope Solutions

sales@m-shot.com中文版
Home > Support > Customer Service Stories

Dual fluorescence FITC -DAPI microscope observation

Date:2016-12-29

Fluorescein FITC (fluorescein isothiocyanate) is one of the most common fluorescence reagent for wide-range biological fluorescence application. The FITC excitation spectrum peak wavelengths is approximately 495nm, and emission spectrum peak wavelengths is approximately 519 nm. That feature makes it efficiency excited by blue light from a fluorescence microscope and represent green color, the filter set is usually 460nm~490nm.


Fluorescence DAPI can pass through an intact cell membrance and show out extremly blue color. It is extensively used in fluorescence microscopy observation to stain both of live and fixed cells. DPAI's spectrum wavelength makes it can be excited by UV light.


MSHOT fluorescence microscope MF31 is an epi fluorescence microscope with LED light source for both of bright field and epi fluorescence. The machine can maximum hold three sets fluorescence filter including blue, green and UV. Observation tube separates the light 100% pass through objectives to reach sample, or 100% light for photography througth trinocular head. The MF31 fluorescence microscope can fit with different sensor size microscope camera to shoot image for different field of view requirement.

 

Use with MShot MS60 sCMOS camera (6.3MP, 1/1.8 inch sensor)

40X objective nerve cell stained by FITC

 

  

40X objective nerve cell stained by DAPI

 

Use with MShot MS23 sCMOS camera (2.3MP, 1/1.2 inch sensor, high sensitivity for weak fluorescence)

 

40X objective nerve cell stained by FITC

 

40X objective nerve cell stained by DAPI

Share it to :

Related News

> New MSHOT Digital Imaging Software
Launched

Microscopy Imaging Expert

Copyright © 2014 Guangzhou Micro-shot Technology Co., Ltd. All rights reserved.